The PfXyn5 has a molecular mass of ~ 55 kDa and showed broad activity against a range of substrates like xylan, xyloglucan, laminarin and p-nitrophenyl-β-d-xylopyranoside, with the highest specific activity of 0.7 U/mg against xylan at pH 4.5 and 50 ☌. funiculosum (PfXyn5) in Pichia pastoris, which was earlier found in our study to be highly implicated in SCB saccharification. Here, we recombinantly expressed and characterized unknown GH5 xylanase from P. The production of second-generation fuels from lignocellulosic residues such as sugarcane bagasse (SCB) requires the synergistic interaction of key cellulose-degrading enzymes and accessory proteins for their complete deconstruction to useful monomeric sugars. The species therefore, has potential for pulp and paper production, but further studies on its chemical consumption levels and other properties need to be ascertained towards a well-informed decision. In appraising the wood for paper production, derived fibre indices/values viz Runkel ratio (ranging from 0.19 to 0.28), flexibility coefficient (ranging from 0.78 to 0.83), and slenderness ratio (ranging from 38.98 to 51.72) appeared to provide some positive indications. Fibre lengths were generally short (<1.6 mm or 1600 μm) with variations in fibre morphology being significant axially than radially. Age and wood position generally had some significant influence on cell characteristics.
Cell characteristics for all ages and tree positions appeared similar but quantitatively, cells in 9-year-old generally exhibited some significant (p <. Data were subjected to statistical analysis using SPSS version 17.00. Sectioning and maceration processes were adopted. This study sought to appraise 7-year and 9-year-old Cedrela odorata for pulp and paper making. Results obtained from confocal microscopy were further confirmed by using TEM–EDXA, indicating that the ratio of lignin concentration in CCML, ML and S2 is 1.5:1.2:1. TEM–EDXA was applied to determine the lignin concentration in the cell corner of middle lamellar (CCML), compound middle lamellae (CML) and S2 layer. Weakly lignified fibres (F) and strongly ligninfied vessels (V) were also detected by using CLSM. Korshinskii showed that strongly lignified CCML, and weakly lignified ML and S2 layer. Korshinskii was typically divided into three layers including the primary wall (P), the middle lamellar (ML) and the secondary wall (S1, S2 and S3). Lignin distribution was determined by using confocal laser scanning microscopy (CLSM) and transmission electron microscopy with energy dispersive X-ray analysis (TEM–EDXA). Anatomy and ultrastructure in cell wall of Caragana Korshinskii was examined by transmission electron microscopy (TEM).
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